Cytokeratin 7 antibody (FITC)

Catalog No. ABIN317491

This Mouse Monoclonal antibody specifically detects Cytokeratin 7 in FACS, IHC (p) and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for Cytokeratin 7 antibody (FITC) (ABIN317491)

Target: Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 7 antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: LP5K

Product Details anti-Cytokeratin 7 Antibody (FITC)

Specificity: The antibody reacts with Cytokeratin 7 which has been found in columnar and glandular epithelium of the lung, cervix, breast, bile ducts and larger collecting ducts of the kidney. Occasionally staining of blood vessel cell walls, particularly endothelial cells, may be observed.

Characteristics: Synonyms: KRT7, KRT-7, SCL, CK-7, CK7, Keratin-7, K7, K-7, Sarcolectin, Keratin type II cytoskeletal 7

Purification: Purified IgG prepared by ammonium sulphate precipitation followed by ion exchange chromatography then conjugated to FITC

Immunogen: Cytoskeletal proteins from cultured HeLa cells

Isotype: IgG2b

Application Details

Application Notes: Immunohistochemistry on frozen sefctions. Immunohistochemistry on paraffin sections (requires protein digestion pre-treatment e. g. trypsin or pronase). Histology positive control: Human skin.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS, 0.09 % Sodium Azide, 0.1 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2 - 8 °C up to one month or (in aliquots) at -20 °C forlonger. Avoid repeated freezing and thawing. Should this product contain a precipitate werecommend microcentrifugation before use.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Cytokeratin 7

Target: Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))

Alternative Name: Cytokeratin 7

Background: Cytokeratin 7 is a 54kD intermediate filament protein found in a variety of glandularepithelia. Cytokeratin 7 has been found in columnar and glandular epithelium of the lung, cervix, breast, bile ducts and larger collecting ducts of the kidney. It is present in the transitional epithelium of bladder as well as ovarian and lungepithelia, and occasionally staining of blood vessel cell walls, particularly endothelial cells, may be observed. However, Cytokeratin 7 is not expressed by epithelial cells of the gastrointestinal tract, colon or prostate. Keratin 7 is often co expressed with keratin 19.Synonyms: CK-7, CK7, K-7, K7, KRT-7, KRT7, Keratin type II cytoskeletal 7, Keratin-7, SCL, Sarcolectin

Gene ID: 3855

UniProt: P08729